Beef and Raw and Liquid Chromatography

doi: x.5625/lar.2011.27.one.37. Epub 2011 Mar 25.

Determination of Oxyclozanide in Beefiness and Milk using High-Functioning Liquid Chromatography Organisation with UV Detector

Affiliations

• PMID: 21826158
• PMCID: PMC3145990
• DOI: 10.5625/lar.2011.27.1.37

Free PMC commodity

Decision of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector

Kyul Jo  et al. Lab Anim Res. 2011 Mar .

Free PMC article

Abstract

This study was developed and validated for the decision of oxyclozanide rest concentrations in beef and commercial milk, using loftier-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C(18), 4.six×250 mm, 5 µm) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (lx:40, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acrid in acetonitrile for commercial milk, dehydration past adding sodium sulfate to the liquid belittling sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 µm syringe filter. This method had good selectivity and recovery (70.lxx±7.90-110.79±fourteen.95%) from the matrices. The LOQs ranged from ix.vii to 9.8 µg/kg for beefiness and commercial milk. The recoveries met the standards gear up by the CODEX guideline.

Keywords: Determination; HPLC; beef; milk; oxyclozanide.

Figures

Figure 1

Chromatogram of oxyclozanide standard (25 ng).

Effigy two

Chromatogram of blank sample of beefiness and spiked sample of beefiness (oxyclozanide 50 ng).

Figure 3

Chromatogram of blank sample of milk and spiked sample of milk (oxyclozanide 25 ng).

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Source: https://pubmed.ncbi.nlm.nih.gov/21826158/

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